Our DNA sequencing
service guarantees fast and excellent results with long read length
and high accuracy. Dideoxy-sequencing is performed from plasmid
templates and directly from PCR-products using the Molecular Devices
MegaBace 1000 fluorescent based capillary DNA Analysis System.
Read lengths are typically 700-900 bases with >98,5% accuracy.
Results
will be reported routinely as chromatograms and base-calls by email
attachment in the format of your choice.
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Plasmid Sequencing
Plasmid sequencing service is performed for common
vectors like e.g. M13, T7, T3, SP6 and CMV with standard sequencing
primers.
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For other vectors
a setup fee of 40 € is charged for the design of appropriate
primers with the first order. If required forward and reverse sequencing
of your insert can be provided.
Direct Sequencing
For your convenience we offer direct sequencing starting from
genomic DNA. Save time and money: Let our experts design PCR
and sequencing primers for optimal results.
If you perform the
PCR yourself you can send us your PCR products and you will get
the sequencing results within 24 hours. Please
note that we need either one or both PCR-primers for sequencing
and additional sequencing primers if the PCR-product is >1200
bp. For the design and the synthesis of appropriate sequencing
primers we will charge 40 €.
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High Throughput
If you are planning a high throughput sequencing project, please
contact us for details. We will be happy to support your R&D
project with our experience and our state of the art sequencing
equipment.
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Bioinformatic
Services
Sequencing results will be reported routinely as chroma-
tograms and base-calls by email attachment in the format of your choice.
Alignments,
data base searches, and other additional bioinformatic services can
be offered upon request.
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